Program: SOAPdenovo2 v2.40 Comments: SOAP has been in evolution from a single alignment tool to a tool package that provides full solution to next generation sequencing data analysis. Currently, it consists of a new alignment tool (SOAPaligner/soap2), a re-sequencing consensus sequence builder (SOAPsnp), an indel finder ( SOAPindel ), a structural variation scanner ( SOAPsv ) and a de novo short reads assembler ( SOAPdenovo ). And a GPU-accelerated alignment tool (SOAP3/GPU) are being implemented. SOAPdenovo is a novel short-read assembly method that can build a de novo draft assembly for the human-sized genomes. The program is specially designed to assemble Illumina GA short reads. It creates new opportunities for building reference sequences and carrying out accurate analyses of unexplored genomes in a cost effective way. Now the new version is available. SOAPdenovo2, which has the advantage of a new algorithm design that reduces memory consumption in graph construction, resolves more repeat regions in contig assembly, increases coverage and length in scaffold construction, improves gap closing, and optimizes for large genome. Source: http://soap.genomics.org.cn/soapdenovo.html http://sourceforge.net/projects/soapdenovo2/files/SOAPdenovo2/ Cite: Li, R., Yu, C., Li, Y., Lam, T.-W., Yiu, S.-M., Kristiansen, K., & Wang, J. (2009). SOAP2: an improved ultrafast tool for short read alignment. Bioinformatics (Oxford, England), 25(15), 1966–7. doi:10.1093/bioinformatics/btp336 Luo, R., Liu, B., Xie, Y., Li, Z., Huang, W., Yuan, J., … Wang, J. (2012). SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. GigaScience, 1(1), 18. doi:10.1186/2047-217X-1-18 Compilation notes: Compilation is trivial following instructions that came with the source code. Basically, you compile with make command and them put the executables in the desirable directory.